@article{83821,
  keywords = {Animals, signal transduction, Microscopy, Fluorescence, Mice, Cell Size, Cytoskeleton, Fibronectins, Extracellular Matrix, Cell Adhesion, Wound Healing, Fibrin, 3T3 Cells, Protein Kinase C},
  author = {Corbett and Wilson and Schwarzbauer},
  title = {Changes in cell spreading and cytoskeletal organization are induced by adhesion to a fibronectin-fibrin matrix.},
  abstract = {<p>Plasma fibronectin (pFN) cross-linked to fibrin during the injury response provides a provisional matrix required for cells to begin tissue repair. Using a synthetic matrix of pFN and fibrin as a substrate for cell adhesion and spreading, we have determined that pFN covalently cross-linked to fibrin into a complex multimer is functionally distinct from pFN immobilized onto a plastic surface. NIH-3T3 cells on a FN-fibrin matrix reach 50\% of the maximal cell area of cells spread on FN-coated plastic. They neither attach nor spread on cross-linked fibrin alone. Cells on pFN-fibrin matrices form few prominent stress fibers and exhibit clear differences in membrane ruffling and filopodial extension when stained with rhodamine-labeled phalloidin. Interestingly, these differences are enhanced by upregulation of protein kinase C. These data suggest that cell-FN interactions can be modified by the molecular context of the protein within the extracellular matrix resulting in distinct cell morphology and cytoskeletal organization.</p>
},
  year = {1996},
  journal = {Blood},
  volume = {88},
  pages = {158-66},
  month = {07/1996},
  issn = {0006-4971},
  language = {eng},
}