@article{83811,
  keywords = {Animals, Humans, Actins, Cytoskeleton, Fibronectins, Focal Adhesion Kinase 1, Cell Adhesion Molecules, CHO Cells, Cricetinae, Focal Adhesion Protein-Tyrosine Kinases, Receptors, Fibronectin, Protein-Tyrosine Kinases},
  author = {Sechler and Schwarzbauer},
  title = {Coordinated regulation of fibronectin fibril assembly and actin stress fiber formation.},
  abstract = {<p>Assembly of a fibronectin (FN) matrix is a multistep process which influences a number of cellular functions including intracellular cytoskeletal organization and signaling responses. We have previously reported on a recombinant FN (recFN), FN delta III1-7, which differs from native FN in its rate of fibril formation. To determine the intracellular consequences of a delay in assembly, we compared the distribution of cytoskeletal proteins during the formation of native and recFN matrices by immunofluorescence at various time points. CHO alpha 5 cell cytoskeleton was reorganized in response to both native and recFN matrix formation. Assembly of native FN induced a rapid reorganization of actin into stress fibers and colocalization of alpha 5 beta 1 integrin, focal adhesion kinase (FAK), vinculin, and paxillin to regions of cell-matrix contact. alpha 5 beta 1 integrins and FAK are also clustered upon binding of FN delta III1-7 to cells but actin reorganization and focal adhesion formation are delayed and appear to be dependent on the formation of FN delta III1-7 fibrils. These results suggest that the structural framework of the matrix plays an important role in the ability of FN to initiate intracellular responses.</p>
},
  year = {1997},
  journal = {Cell Adhes Commun},
  volume = {4},
  pages = {413-24},
  month = {03/1997},
  issn = {1061-5385},
  language = {eng},
}