@article{83626,
  keywords = {Animals, polymers, Surface Properties, Oligopeptides, Mice, Cell Adhesion, NIH 3T3 Cells, Caprolactam, Cell Adhesion Molecules, Organometallic Compounds},
  author = {Joseph Dennes and Geoffrey Hunt and Jean Schwarzbauer and Jeffrey Schwartz},
  title = {High-yield activation of scaffold polymer surfaces to attach cell adhesion molecules.},
  abstract = {<p>Zirconium tetra(tert-butoxide) reacts with surface amide groups of polyamide nylon 6/6 to give (eta(2)-amidate)zirconium complexes in high yield. These surface complexes react to bond the cell-adhesive peptide arginine-glycine-aspartic acid (RGD) to the polymer surface. A surface loading of 0.18 nmol/cm(2) of RGD is achieved, which is 20-1000 times higher than previously reported attainable on natural or synthetic polymers by other strategies. Approximately 40\% of the nylon surface is covered by the RGD, which gives a surface that is both stable to hydrolysis and highly active for cell adhesion and spreading in vitro.</p>
},
  year = {2007},
  journal = {J Am Chem Soc},
  volume = {129},
  pages = {93-7},
  month = {01/2007},
  issn = {0002-7863},
  doi = {10.1021/ja065217t},
  language = {eng},
}