@article{83561,
  keywords = {Animals, microscopy, Recombinant Proteins, Caenorhabditis elegans, Green Fluorescent Proteins, Phenotype, Cell Movement, Animals, Genetically Modified, Transfection, Caenorhabditis elegans Proteins, RNA Interference, Gene Knockdown Techniques, Cell Migration Assays, Larva},
  author = {Ming-Ching Wong and Maria Martynovsky and Jean Schwarzbauer},
  title = {Analysis of cell migration using Caenorhabditis elegans as a model system.},
  abstract = { The nematode Caenorhabditis elegans is an excellent model system in which to study long-distance cell migration in vivo. This chapter describes methods used to study a subset of migratory cells in the hermaphrodite nematode, the distal tip cells. These methods take advantage of the organism{\textquoteright}s transparent body and the expression of green fluorescent protein to observe cell migration and behavior. Additionally, the availability of nematode mutants and gene knockdown techniques that affect cell migration allow the analysis and comparison of wild-type and aberrant migratory paths. Methods for nematode growth and maintenance, strain acquisition, observation and live imaging, gene knockdown, and analysis of cell migration defects are covered. },
  year = {2011},
  journal = {Methods Mol Biol},
  volume = {769},
  pages = {233-47},
  issn = {1940-6029},
  doi = {10.1007/978-1-61779-207-6_16},
  language = {eng},
}