@article{83511,
  keywords = {Animals, Caenorhabditis elegans, Cell Movement, Integrins, Caenorhabditis elegans Proteins, Cell Adhesion, Gonads},
  author = {Ming-Ching Wong and William Kennedy and Jean Schwarzbauer},
  title = {Transcriptionally regulated cell adhesion network dictates distal tip cell directionality.},
  abstract = {<p>BACKGROUND: The mechanisms that govern directional changes in cell migration are poorly understood. The migratory paths of two distal tip cells (DTC) determine the U-shape of the C. elegans hermaphroditic gonad. The morphogenesis of this organ provides a model system to identify genes necessary for the DTCs to execute two stereotyped turns. RESULTS: Using candidate genes for RNAi knockdown in a DTC-specific strain, we identified two transcriptional regulators required for DTC turning: cbp-1, the CBP/p300 transcriptional coactivator homologue, and let-607, a CREBH transcription factor homologue. Further screening of potential target genes uncovered a network of integrin adhesion-related genes that have roles in turning and are dependent on cbp-1 and let-607 for expression. These genes include src-1/Src kinase, tln-1/talin, pat-2/α integrin and nmy-2, a nonmuscle myosin heavy chain. CONCLUSIONS: Transcriptional regulation by means of cbp-1 and let-607 is crucial for determining directional changes during DTC migration. These regulators coordinate a gene network that is necessary for integrin-mediated adhesion. Overall, these results suggest that directional changes in cell migration rely on the precise gene regulation of adhesion.</p>
},
  year = {2014},
  journal = {Dev Dyn},
  volume = {243},
  pages = {999-1010},
  month = {08/2014},
  issn = {1097-0177},
  doi = {10.1002/dvdy.24146},
  language = {eng},
}